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首頁 > 產品中心 > 細胞生物學 > 重組蛋白 > abs04030Recombinant Human CD99/MIC2 （C-Fc）

Recombinant Human CD99/MIC2 （C-Fc）

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  • 產品型號：abs04030
  • 廠商性質：生產廠家
  • 更新時間：2025-02-07
  • 訪  問  量：1316

詳細介紹

品牌absin規格10ug
供貨周期一周
概述
描述Recombinant Human CD99 is produced by our Mammalian expression system and the target gene encoding Asp23-Asp122 is expressed with a Fc tag at the C-terminus.
別名CD99 Antigen, 12E7, E2 Antigen, Protein MIC2, T-Cell Surface Glycoprotein E2, CD99, MIC2, MIC2X, MIC2Y
形態Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
來源Human Cells
氨基酸序列DGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSS GSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADVDDIEGRMDEPKSCDKTHTCPPCPAPELLG GPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYR VVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLT CLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWGNVFSCSVMHEA LHNHYTQKSLSLSPGK
內毒素水平Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Accession #P14209
性能
背景CD99 is a type I transmembrane glycoprotein and the founding member of the CD99 family of molecules. The extracellular domain of CD99 contains no identifiable motifs, its cytoplasmic region, although short, does have signal transduction capability. Cells known to express CD99 include fibroblasts, neutrophils, T cells, double positive thymocytes, CD34+ stem cells, monocytes and endothelial cells. Two types of CD99 isoforms have been classified. Native human CD99 is referred to as the long, or type I isoform. The best studied type II isoform shows an Asp-Gly substitution for the C terminal 27 amino acids. The type I and II isoforms have distinctive signal transduction pathways (FAKsrc for type I PI3K plus srcERK1/2 for type II), and mediate clearly different biological outcomes. Homophilic interaction between CD99 on the neutrophil and CD99 on the endothelial cell regulates the transendothelial migration of neutrophils during inflammation. Human CD99 has 48% aa sequence identity to mouse CD99.
溶解方法Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
純度Greater than 95% as determined by reducing SDS-PAGE.

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