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首頁 > 產品中心 > 細胞生物學 > 重組蛋白 > abs040155'-NT/CD73 （C-6His）

5'-NT/CD73 （C-6His）

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  • 產品型號：abs04015
  • 廠商性質：生產廠家
  • 更新時間：2025-02-07
  • 訪  問  量：1308

詳細介紹

品牌absin規格10ug
供貨周期一周
概述
描述Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
別名5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, CD73, NT5E, NT5, NTE
形態Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
來源Human Cells
氨基酸序列WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVIRRAEPNVLLLDAGDQYQGTI WFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISG LYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHS GFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKY LGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQ SCRFRECNMGNLICDAMINNNLRHADETFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLP FGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRV PSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRI KVDHHHHHH
內毒素水平Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Accession #P21589
性能
背景CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
保存方法Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
純度Greater than 95% as determined by reducing SDS-PAGE.

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