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首頁 > 產品中心 > 細胞生物學 > 重組蛋白 > abs04207HDGF （C-6His）（10ug）

HDGF （C-6His）（10ug）

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  • 產品型號：abs04207
  • 廠商性質：生產廠家
  • 更新時間：2025-02-07
  • 訪  問  量：1154

詳細介紹

品牌absin貨號abs04207
規格10ug供貨周期一周
概述
描述Recombinant Human Hepatoma-Derived Growth Factor is produced by our E.coli expression system and the target gene encoding Met1-Tyr100 is expressed with a 6His tag at the C-terminus.
別名Hepatoma-Derived Growth Factor, HDGF, High Mobility Group Protein 1-Kike 2, HMG-1L2, HDGF, HMG1L2
形態Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 1mM DTT, 1mM EDTA, pH 7.5.
來源E. coli
氨基酸序列MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFP YEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYLEHHHHHH
內毒素水平Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Accession #P51858
性能
背景Hepatoma-Derived Growth Factor is a original member of the HDGF family. HDGF is a cytoplasmic protein and contains one PWWP domain. HDGF expression levels are high in the nucleus and cytoplasm of smooth muscle and endothelial cells. HDGF has proliferative, angiogenic and neurotrophic activity. HDGF was initially characterized as a secreted mitogen from the Huh-7 human hepatoma cell line. As a heparin-binding protein, which is highly expressed in tumor cells where it stimulates proliferation. HDGF has mitogenic activity for fibroblasts and acts as a transcriptional repressor. It has been shown that HDGF is linked with tumorigenesis and the growth of cancer.
溶解方法Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
純度Greater than 95% as determined by reducing SDS-PAGE.

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